Composite

Part:BBa_K1044005:Design

Designed by: Koki Tsutsumi,Eshin Mitsui   Group: iGEM13_Biwako_Nagahama   (2013-09-05)


Cellulose synthased operon (CelA,B,C) from Agrobacterium tumefaciens C58.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 594
    Illegal BamHI site found at 4975
    Illegal XhoI site found at 3702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 643
    Illegal NgoMIV site found at 1526
    Illegal NgoMIV site found at 2291
    Illegal NgoMIV site found at 2530
    Illegal NgoMIV site found at 2587
    Illegal NgoMIV site found at 2922
    Illegal NgoMIV site found at 3231
    Illegal AgeI site found at 2492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 661
    Illegal BsaI site found at 886
    Illegal BsaI site found at 1165
    Illegal BsaI site found at 4205
    Illegal BsaI.rc site found at 3987
    Illegal SapI site found at 768
    Illegal SapI.rc site found at 4669
    Illegal SapI.rc site found at 5530


Design Notes

none


Source

writing now

References